BMP4 Antibody (OAGA01420)

Catalog No: OAGA01420

Size:100 ul

BMP4 antibody detects BMP4 protein by Western blot analysis.
A. 30 ug A549 whole cell lysate/extract
10 % SDS-PAGE
BMP4 antibody (OAGA01420) dilution: 1:1000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
10% SDS PAGE
OAGA01420 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft, using BMP4 (OAGA01420) antibody at 1:100 dilution.

Immunofluorescence analysis of methanol-fixed HeLa, using BMP4 (OAGA01420) antibody at 1:100 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid 1X PBS, 1% BSA and 20% glycerol (pH 7) with 0.025% Proclin300
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC-P, WB, ICC
• Reconstitution and Storage: Keep as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze/thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human BMP4. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.36 mg/ml
• Specificity: This antibody is able to recognize full length BMP4 protein (~55 kDa) and N-terminal propeptide BMP4 protein (~40 kDa) but unable to detect mature form.
• Application Info: WB: 1:500~3000
  ICC/IF: 1:100~1000
  IHC-P: 1:100~1000

Target Info

• Gene Symbol: BMP4
• Gene Full Name: bone morphogenetic protein 4
• Alias Symbols: ZYME, BMP2B, OFC11, BMP2B1, MCOPS6
• NCBI Gene Id: 652
• Description of Target: The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.
• Uniprot ID: P12644
• Molecular Weight: 47 kDa