BMP1 ELISA Kit (Mouse) (OKCD02401)

Catalog No: OKCD02401

Size:96WELLS

Mouse BMP1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	96-103%	98-105%	95-104%	91-102%
  EDTA Plasma (n=5)	96-104%	83-98%	96-103%	79-105%
  Heparin Plasma (n=5)	80-102%	82-94%	97-104%	98-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 1 (BMP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 1 (BMP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 1 (BMP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bone Morphogenetic Protein 1 (BMP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	86-95	92
  EDTA Plasma (n=5)	92-101	96
  Heparin Plasma(n=5)	78-94	84

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 1 (BMP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 1 (BMP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-BMP1 Microplate: 96 Wells (12 x 8 Well strips)
  • BMP1 Lyophilized Standard: 2
  • 100X Biotinylated BMP1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 32 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 1 (BMP1). No significant cross-reactivity or interference between Bone Morphogenetic Protein 1 (BMP1) and analogues was observed.
• Publications:

  Bone secreted factors induce cellular quiescence in prostate cancer cells. Sci Rep. 9, 18635 (2019). 31819067

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for BMP1 ELISA kit (Mouse) on CiteAb

Target Info

• Gene Symbol: Bmp1
• Gene Full Name: bone morphogenetic protein 1
• Alias Symbols: bone morphogenetic protein 1;mammalian tolloid protein;Pcp;procollagen C-proteinase;Tld.
• NCBI Gene Id: 12153
• Protein Name: Bone morphogenetic protein 1
• Description of Target: Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD) (By similarity). Responsible for the proteolytic activation of lysyl oxidase LOX.
• Uniprot ID: P98063
• Protein Accession #: NP_033885.2
• Nucleotide Accession #: NM_009755.3
• Protein Size (# AA): 991