Catalog No: OKBB00131
Size:96 Tests
Price: $466.00
SKU
OKBB00131
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's human BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-2 has been precoated onto 96-well plates. Standards(CHO, Q283-R396) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human BMP-2 amount of sample captured in plate.
ELISA Kit Range31.2 pg/ml - 2,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (pg/mL)28574211973068341358
St. Dev.9.1225.9737.1218.9758.3897.78
%CV3.23.53.106.1977.20
ELISA Kit Component
ComponentAmount
Lyophilized recombinant human BMP-2 standard10 ng/tube x 2
Anti-human BMP-2 Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti-human BMP-2 antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Additional InformationRange: 62.5pg/ml-4000pg/ml
::Principle: Aviva’s human BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human BMP-2 specific-specific monoclonal antibodies were precoated onto 96-well plates. The human specific detection monoclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BMP-2 amount of sample captured in plate.
::Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-β) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample Typebone tissue, cell culture supernates and serum
Sensitivity<2 pg/ml
Predicted Homology Based on Immunogen SequenceDetectable cross-reactivity with other BMP <1%.
Reference1. Gopal Rao, V. V. N.; Loffler, C.; Wozney, J. M.; Hansmann, I. The gene for bone morphogenetic protein 2A
(BMP2A) is localized to human chromosome 20p12 by radioactive and nonradioactive in situ hybridization. Hum.
Genet. 90: 299-302, 1992.
2. Hallahan, A. R.; Pritchard, J. I.; Chandraratna, R. A. S.; Ellenbogen, R. G.; Geyer, J. R.; Overland, R. P.; Strand, A.
D.; Tapscott, S. J.; Olson, J. M. BMP-2 mediates retinoid-induced apoptosis in medulloblastoma cells through a
paracrine effect. Nature Med. 9: 1033-1038, 2003.
3. Cheng, H.; Jiang, W.; Phillips, F. M.; Haydon, R. C.; Peng, Y.; Zhou, L.; Luu, H. H.; An, N.; Breyer, B.; Vanichakarn,
P.; Szatkowski, J. P.; Park, J. Y.; He, T.-C. Osteogenic activity of the fourteen types of human bone morphogenetic
proteins (BMPs). J. Bone Joint Surg. 85-A: 1544-1552, 2003.
SpecificityNatural and recombinant human BMP-2
ImmunogenExpression system for standard: CHO; Immunogen sequence: Q283-R396
DescriptionFor quantitative detection of human BMP-2 in bone tissue, cell culture supernatants and serum.
Gene SymbolBMP2
Gene Full Namebone morphogenetic protein 2
Alias SymbolsBDA2, BMP-2, BMP-2A, Bmp2, BMP2_HUMAN, BMP2A, Bone morphogenetic protein 2, Bone morphogenetic protein 2A
NCBI Gene Id650
Protein NameBone morphogenetic protein 2
Description of TargetBone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-β) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.
Uniprot IDP12643
Protein Accession #NP_001191.1
Nucleotide Accession #NM_001200.3
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