Catalog No: OKCD02294
Size:96WELLS
Price: $650.00
SKU
OKCD02294
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for Beta-Endorphin ELISA Kit (Rat) (OKCD02294) |
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Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||||
ELISA Kit Duration | 2h | ||||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Beta-Endorphin (bEP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Beta-Endorphin (bEP) and unlabeled Beta-Endorphin (bEP) (Standards or samples) with the pre-coated antibody specific to Beta-Endorphin (bEP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample. | ||||||||||||||||||||||||
ELISA Kit Range | 12.35-1,000pg/mL | ||||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | CAS: 60617-12-1 PubChem: 16132316 | ||||||||||||||||||||||||
Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids | ||||||||||||||||||||||||
Sensitivity | 4.62 pg/mL | ||||||||||||||||||||||||
Assay Info | Assay Methodology: Competitive Inhibition Immunoassay |
Gene Symbol | BEP |
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Alias Symbols | Corticotropin-lipotropin. |
Protein Name | Pro-opiomelanocortin |
Description of Target | Corticotropin: Stimulates the adrenal glands to release cortisol. |
Uniprot ID | P01194 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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