Catalog No: OKCD02294
Size:96WELLS
Price: $650.00
SKU
OKCD02294
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

Beta-Endorphin ELISA Kit (Rat) (OKCD02294)

Datasheets/ManualsPrintable datasheet for Beta-Endorphin ELISA Kit (Rat) (OKCD02294)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)92-103%80-92%86-94%83-101%
EDTA plasma(n=5)88-95%79-103%80-103%94-103%
heparin plasma(n=5)79-99%95-103%98-105%99-105%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Beta-Endorphin (bEP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Beta-Endorphin (bEP) and unlabeled Beta-Endorphin (bEP) (Standards or samples) with the pre-coated antibody specific to Beta-Endorphin (bEP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample.
ELISA Kit Range12.35-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)80-9793
EDTA plasma(n=5)82-9187
heparin plasma(n=5)85-10588
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Beta-Endorphin Microplate96 Wells (12 x 8 Well Strips)
Beta-Endorphin Lyophilized Standard2
Beta-Endorphin Biotin Complex1
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Biotin Complex Reconstitution Buffer1 x 300 uL
Additional InformationCAS: 60617-12-1
PubChem: 16132316
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids
Sensitivity4.62 pg/mL
Assay InfoAssay Methodology: Competitive Inhibition Immunoassay
Gene SymbolBEP
Alias SymbolsCorticotropin-lipotropin.
Protein NamePro-opiomelanocortin
Description of TargetCorticotropin: Stimulates the adrenal glands to release cortisol.
Uniprot IDP01194
Write Your Own Review
You're reviewing:Beta-Endorphin ELISA Kit (Rat) (OKCD02294)
Your Rating