BECN1 ELISA Kit (Rat) (OKEH03267)

Catalog No: OKEH03267

Size:96T

Rat BECN1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology BECN1 ELISA Kit (Rat) (OKEH03267) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Becn1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Becn1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Becn1 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Component:
  • Anti-Becn1 Microplate: 96 Wells (12 x 8 Well strips)
  • Becn1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Becn1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 39 pg/mL
• Specificity: Natural and recombinant Rat Beclin-1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Becn1
• Gene Full Name: beclin 1
• Alias Symbols: beclin 1 (coiled-coil, myosin-like BCL2-interacting protein);beclin 1, autophagy related;beclin-1;Beclin1;coiled-coil myosin-like BCL2-interacting protein;Protein GT197.
• NCBI Gene Id: 114558
• Protein Name: Q91XJ1
• Description of Target: Beclin-1
• Uniprot ID: https://www.uniprot.org/uniprot/Q91XJ1
• Protein Accession #: NP_001029289.1
• Nucleotide Accession #: NM_001034117.1
• Protein Size (# AA): 448
• Molecular Weight: 52 kDa