Catalog No: OKEH03266
Size:96T
Price: $525.00
SKU
OKEH03266
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology BECN1 ELISA Kit (Mouse) (OKEH03266) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Becn1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Becn1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Becn1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.3% (n = 20)
Mean Inter-assay CV%: <= 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Becn1 Microplate96 Wells (12 x 8 Well strips)
Becn1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Becn1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Mouse Beclin-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBecn1
Gene Full Namebeclin 1, autophagy related
Alias SymbolsAtg;Atg6;Bcl-2-interacting protein beclin;Beclin-1;Coiled-coil myosin-like BCL2-interacting protein;coiled-coil, myosin-like BCL2-interacting protein.
NCBI Gene Id56208
Protein NameO88597
Description of TargetBeclin-1
Uniprot IDhttps://www.uniprot.org/uniprot/O88597
Protein Accession #NP_062530.2
Nucleotide Accession #NM_019584.3
Protein Size (# AA)448
Molecular Weight52 kDa
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