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Catalog No: OKEH04415 (Formerly GWB-DJE0KK)
Size:96T
Price: $550.00
SKU
OKEH04415
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH04415
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology BECN1 ELISA Kit (Human) (OKEH04415) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BECN1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for BECN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BECN1 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.2% (n = 20)
Mean Inter-assay CV%: <= 9.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-BECN1 Microplate96 Wells (12 x 8 Well Strips)
BECN1 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
BECN1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Beclin-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBECN1
Gene Full Namebeclin 1
Alias SymbolsATG6;ATG6 autophagy related 6 homolog;beclin 1 (coiled-coil, moesin-like BCL2-interacting protein);beclin 1, autophagy related;beclin1;beclin-1;coiled-coil myosin-like BCL2-interacting protein;Protein GT197;testis secretory sperm-binding protein Li 215e;VPS30.
NCBI Gene Id8678
Protein NameQ14457
Description of TargetBeclin-1
Uniprot IDhttps://www.uniprot.org/uniprot/Q14457
Protein Accession #NP_001300927.1
Nucleotide Accession #NM_001313998.1
Protein Size (# AA)450
Molecular Weight52 KDa
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