Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH02400
Size:96T
Price: $475.00
SKU
OKEH02400
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

BCL11B ELISA Kit (Human) : 96 Wells (OKEH02400)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology BCL11B ELISA Kit (Human) (OKEH02400) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCL11B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCL11B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCL11B captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 7.8% (n = 20)
Mean Inter-assay CV%: < 11.6% (n = 20)
ELISA Kit Component
ComponentAmount
BCL11B Microplate96 Wells (12 x 8 Well strips)
BCL11B Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated DTNBP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity23 pg/mL
SpecificityNatural and recombinant Human B-cell lymphoma/leukemia 11B
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBCL11B
Gene Full NameBAF chromatin remodeling complex subunit BCL11B
Alias SymbolsATL1;ATL1-alpha;ATL1-beta;ATL1-delta;ATL1-gamma;B cell CLL/lymphoma 11B;B-cell CLL/lymphoma 11B;B-cell CLL/lymphoma 11B (zinc finger protein);B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein;B-cell lymphoma/leukaemia 11B;B-cell lymphoma/leukemia 11B;BCL-11B;BCL11B, BAF complex component;BCL11B/TRDC fusion;COUP-TF-interacting protein 2;CTIP2;CTIP-2;hRit1;hRIT1-alpha;IDDFSTA;IMD49;radiation-induced tumor suppressor gene 1 protein;RIT1;SMARCM2;zinc finger protein hRit1 alpha;ZNF856B.
NCBI Gene Id64919
Protein NameQ9C0K0
Description of TargetB-cell lymphoma/leukemia 11B
Uniprot IDhttps://www.uniprot.org/uniprot/Q9C0K0
Protein Accession #NP_001269166.1
Nucleotide Accession #NM_001282237.1
Protein Size (# AA)894
Molecular Weight96 kDa
Write Your Own Review
You're reviewing:BCL11B ELISA Kit (Human) : 96 Wells (OKEH02400)
Your Rating
We found other products you might like!