BCL-2 Antibody (Phospho-Thr69) (OAAF07514)

Catalog No: OAAF07514 (Formerly GWB-ASB323)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using BCL-2 (Phospho-Thr69) Antibody. The picture on the right is blocked with the phospho peptide.

Immunofluorescence analysis of HeLa cells, using BCL-2 (Phospho-Thr69) Antibody. The picture on the right is blocked with the phospho peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin
• Additional Information: Modification Sites: Human:T69
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human BCL-2 around the phosphorylation site of Thr69.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Peptide Sequence: Synthetic peptide located within the following region: PGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHL
• Concentration: 1mg/ml
• Specificity: BCL-2 (Phospho-Thr69) Antibody detects endogenous levels of BCL-2 only when phosphorylated at Thr69.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Application Info:
  IHC: 1:50~1:100
  IF: 1:100~1:500
  ELISA: 1:20000

Target Info

• Gene Symbol: AKT3|BCL2
• Gene Full Name: AKT serine/threonine kinase 3|BCL2 apoptosis regulator
• Alias Symbols: apoptosis regulator Bcl-2;B-cell CLL/lymphoma 2;Bcl-2;MPPH;MPPH2;PKB gamma;PKBG;PKB-GAMMA;PPP1R50;PRKBG;protein phosphatase 1, regulatory subunit 50;RAC-gamma;RAC-gamma serine/threonine protein kinase;RAC-gamma serine/threonine-protein kinase;RAC-PK-gamma;STK-2;v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma).
• NCBI Gene Id: 10000|596
• Protein Name: Apoptosis regulator Bcl-2
• Description of Target: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
• Uniprot ID: P10415
• Molecular Weight: 26 kDa