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Datasheets/Manuals | Printable datasheet for BCL-2 Antibody (Phospho-Ser70) (OAAF07688) |
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Predicted Species Reactivity | Human|Monkey |
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Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot |
Additional Information | Modification Sites: Human:S70 |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human BCL-2 around the phosphorylation site of Ser70. |
Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
Peptide Sequence | Synthetic peptide located within the following region: GAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVP |
Concentration | 1mg/ml |
Specificity | BCL-2 (Phospho-Ser70) Antibody detects endogenous levels of BCL-2 only when phosphorylated at Ser70. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Application Info | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 |
Gene Symbol | AKT3|BCL2 |
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Gene Full Name | AKT serine/threonine kinase 3|BCL2 apoptosis regulator |
Alias Symbols | apoptosis regulator Bcl-2;B-cell CLL/lymphoma 2;Bcl-2;MPPH;MPPH2;PKB gamma;PKBG;PKB-GAMMA;PPP1R50;PRKBG;protein phosphatase 1, regulatory subunit 50;RAC-gamma;RAC-gamma serine/threonine protein kinase;RAC-gamma serine/threonine-protein kinase;RAC-PK-gamma;STK-2;v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma). |
NCBI Gene Id | 10000|596 |
Protein Name | Apoptosis regulator Bcl-2 |
Description of Target | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). |
Uniprot ID | P10415 |
Molecular Weight | 26 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "BCL-2 Antibody (Phospho-Ser70) (OAAF07688)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Monkey".
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How long will it take to receive "BCL-2 Antibody (Phospho-Ser70) (OAAF07688)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "BCL-2 Antibody (Phospho-Ser70) (OAAF07688)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "BCL-2 Antibody (Phospho-Ser70) (OAAF07688)"?
This target may also be called "apoptosis regulator Bcl-2;B-cell CLL/lymphoma 2;Bcl-2;MPPH;MPPH2;PKB gamma;PKBG;PKB-GAMMA;PPP1R50;PRKBG;protein phosphatase 1, regulatory subunit 50;RAC-gamma;RAC-gamma serine/threonine protein kinase;RAC-gamma serine/threonine-protein kinase;RAC-PK-gamma;STK-2;v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)." in publications.
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What is the shipping cost for "BCL-2 Antibody (Phospho-Ser70) (OAAF07688)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "BCL-2 Antibody (Phospho-Ser70) (OAAF07688)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "BCL-2 Antibody (Phospho-Ser70) (OAAF07688)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "26 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "BCL-2 Antibody (Phospho-Ser70) (OAAF07688)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "AKT3|BCL2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "AKT3|BCL2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "AKT3|BCL2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "AKT3|BCL2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "AKT3|BCL2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "AKT3|BCL2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.