Catalog No: OKCD01123 (Formerly GWB-KBBTT2)
Size:96WELLS
Price: $725.00
SKU
OKCD01123
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for BCKDK ELISA Kit (Rat) (OKCD01123) |
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Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 3.12-200ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 1.29 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK). No significant cross-reactivity or interference between Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | Bckdk |
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Gene Full Name | branched chain ketoacid dehydrogenase kinase |
Alias Symbols | [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial;BCKDHKIN;BCKD-kinase;branched-chain alpha-ketoacid dehydrogenase kinase. |
NCBI Gene Id | 29603 |
Protein Name | [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial |
Description of Target | Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex. |
Uniprot ID | Q00972 |
Protein Accession # | NP_062117 |
Nucleotide Accession # | NM_019244.2 |
Protein Size (# AA) | 412 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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