Catalog No: OKCD02398
Size:96WELLS
Price: $675.00
SKU
OKCD02398
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Datasheets/ManualsPrintable datasheet for BCAR3 ELISA Kit (Human) (OKCD02398)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)80-99%94-102%90-102%86-97%
EDTA Plasma (n=5)97-105%94-102%87-96%79-103%
Heparin Plasma (n=5)97-105%87-101%97-104%93-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Breast Cancer Anti-Estrogen Resistance 3 (BCAR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Breast Cancer Anti-Estrogen Resistance 3 (BCAR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Breast Cancer Anti-Estrogen Resistance 3 (BCAR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78.12-5000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)88-10596
EDTA Plasma (n=5)85-9289
Heparin Plasma(n=5)91-10497
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-BCAR3 Microplate96 Wells (12 x 8 Well strips)
BCAR3 Lyophilized Standard2
100X Biotinylated BCAR3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity27 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Breast Cancer Anti-Estrogen Resistance 3 (BCAR3). No significant cross-reactivity or interference between Breast Cancer Anti-Estrogen Resistance 3 (BCAR3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolBCAR3
Gene Full NameBCAR3 adaptor protein, NSP family member
Alias SymbolsAND-34;BCAR3, NSP family adaptor protein;breast cancer anti-estrogen resistance 3;breast cancer antiestrogen resistance 3 protein;breast cancer anti-estrogen resistance protein 3;dJ1033H22.2 (breast cancer anti-estrogen resistance 3);epididymis secretory sperm binding protein;mig-7;MIG7;migration inducting gene-7;novel SH2-containing protein 2;NSP2;SH2 domain-containing protein 3B;SH2D3B.
NCBI Gene Id8412
Protein NameBreast cancer anti-estrogen resistance protein 3
Description of TargetMay act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins.
Uniprot IDO75815
Protein Accession #NP_001248337.1
Nucleotide Accession #NM_001261408.1
Protein Size (# AA)825
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