Bax Antibody (OAPB00247)

Catalog No: OAPB00247 (Formerly GWB-6E4ABF)

Size:100UG

Western blot analysis of Bax in HL-60 cell lysates with Bax antibody at (A) 1, (B) 2, and (C) 4 μg/mL.

Immunofluorescence of Bax in HL60 cells with Bax antibody at 2 ug/mL.

Immunocytochemistry staining of HL-60 cells using Bax at 2 μg/mL.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse
• Product Format: Liquid. Bax Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunoprecipitation|Western blot
• Additional Information: HL60 Cell Lysate (OCPB00173)
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: Bax antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Bax antibody was raised against a peptide corresponding to 16 amino acids near the amino-terminus of human Bax. The immunogen is located within the first 50 amino acids of Bax.
• Purification: Ion Exchange
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-Bax antibody is Catalog # OEPB02341
• Application Info: Bax antibody can be used for detection of Bax by Western blot at 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.;Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.;Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.;Oltvai ZN, Milliman CL, and Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 1993; 74:609-19.

Target Info

• Gene Symbol: BAX
• Gene Full Name: BCL2 associated X, apoptosis regulator
• Alias Symbols: apoptosis regulator BAX;Baxdelta2G9;Baxdelta2G9omega;Baxdelta2omega;BCL2 associated X protein;BCL2-associated X protein omega;bcl2-L-4;BCL2L4;bcl-2-like protein 4.
• NCBI Gene Id: 581
• Protein Name: Apoptosis regulator BAX|Bax alpha [Homo sapiens]
• Description of Target: Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
• Uniprot ID: Q07812
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/AAA03619