BAG2 Antibody (OABB02007)

Catalog No: OABB02007

Size:100UG

Anti-BAG2 Picoband antibody, OABB02007, Western blotting
All lanes: Anti BAG2 (OABB02007) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Predicted bind size: 24KD
Observed bind size: 24KD

Anti-BAG2 Picoband antibody, OABB02007, IHC(P)
IHC(P): Human Pancreatic Cancer Tissue

Anti-BAG2 Picoband antibody, OABB02007, IHC(P)
IHC(P): Mouse Testis Tissue

Anti-BAG2 Picoband antibody, OABB02007, IHC(P)
IHC(P): Rat Testis Tissue

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Human
• Product Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Flow cytometry|Immunocytochemistry|Immunofluorescence|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  Background: BAG family molecular chaperone regulator 2 is a protein that in humans is encoded by the BAG2 gene. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. The functional antagonisms displayed between BAG family proteins and Hip suggest that a proper balance of these 2 types of protein is required for achieving optimal cycles of substrate binding and release required for inducting conformational changes in proteins, with Hip promoting peptide substrate binding by Hsc70/Hsp70 and BAG family proteins promoting dissociation.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: E.coli-derived human BAG2 recombinant protein (Position: M1-N211). Human BAG2 shares 93.4% amino acid (aa) sequence identity with mouse BAG2.
• Purification: Affinity Purified
• Peptide Sequence: Synthetic peptide located within the following region: MAQAKINAKANEGRFCRSSSMADRSSRLLESLDQLELRVEALREAATAVEQEKEILLEMIHSIQNSQDMRQISDGEREELNLTANRLMGRTLTVEVSVETIRNPQQQESLKHATRIIDEVVNKFLDDLGNAKSHLMSLYSACSSEVPHGPVDQKFQSIVIGCALEDQKKIKRRLETLLRNIENSDKAIKLLEHSKGAGSKTLQQNAESRFN
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Mouse, Rat: By Heat
• Reference: 1. Takayama S, Xie Z, Reed JC (Feb 1999). "An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators". J Biol Chem 274 (2): 781–6.
  2. "Entrez Gene: BAG2 BCL2-associated athanogene 2".
  
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for BAG family molecular chaperone regulator 2(BAG2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Target Info

• Gene Symbol: BAG2
• Gene Full Name: BAG cochaperone 2
• Alias Symbols: BAG family molecular chaperone regulator 2;BAG-2;BCL2 associated athanogene 2;bcl-2-associated athanogene 2;dJ417I1.2;dJ417I1.2 (BAG-family molecular chaperone regulator 2).
• NCBI Gene Id: 9532
• Protein Name: BAG family molecular chaperone regulator 2
• Description of Target: Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release (PubMed:24318877, PubMed:9873016).
• Uniprot ID: O95816
• Molecular Weight: 23772 MW