Catalog No: OKEH01293
Size:96T
Price: $525.00
SKU
OKEH01293
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology BACE2 ELISA Kit (Human) (OKEH01293) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BACE2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BACE2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BACE2 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 8.0% (n = 20)
ELISA Kit Component
ComponentAmount
BACE2 Microplate96 Wells (12 x 8 Well strips)
BACE2 Lyophilized Standard2
100X Biotinylated BACE2 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Beta-secretase 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBACE2
Gene Full Namebeta-secretase 2
Alias Symbols56 kDa aspartic-like protease;AEPLC;ALP56;ASP1;ASP21;Aspartic-like protease 56 kDa;aspartyl protease 1;BAE2;beta-secretase 2;beta-site amyloid beta A4 precursor protein-cleaving enzyme 2;Beta-site amyloid precursor protein cleaving enzyme 2;beta-site APP-cleaving enzyme 2;CDA13;CEAP1;Down region aspartic protease;Down syndrome region aspartic protease;DRAP;memapsin-1;membrane-associated aspartic protease 1;SLCO3A1/BACE2 fusion;theta-secretase;transmembrane aspartic proteinase Asp1.
NCBI Gene Id25825
Protein NameQ9Y5Z0
Description of TargetBeta-secretase 2
Uniprot IDhttps://www.uniprot.org/uniprot/Q9Y5Z0
Protein Accession #NP_036237.2
Nucleotide Accession #NM_012105.4
Protein Size (# AA)518
Molecular Weight56 kDa
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