BACE1 ELISA Kit (Rat) (OKCD02313)

Catalog No: OKCD02313

Size:96WELLS

Rat BACE1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	94-102%	86-102%	85-94%	92-99%
  EDTA Plasma (n=5)	83-98%	98-105%	78-90%	97-104%
  Heparin Plasma (n=5)	87-96%	85-99%	95-103%	99-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-Site APP Cleaving Enzyme 1 (bACE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-Site APP Cleaving Enzyme 1 (bACE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-Site APP Cleaving Enzyme 1 (bACE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Beta-Site APP Cleaving Enzyme 1 (bACE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	85-92	89
  EDTA Plasma (n=5)	78-99	92
  Heparin Plasma(n=5)	80-97	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Site APP Cleaving Enzyme 1 (bACE1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Site APP Cleaving Enzyme 1 (bACE1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-BACE1 Microplate: 96 Wells (12 x 8 Well strips)
  • BACE1 Lyophilized Standard: 2
  • 100X Biotinylated BACE1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, cerebrospinal fluid and other biological fluids
• Sensitivity: 6.7 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Beta-Site APP Cleaving Enzyme 1 (bACE1). No significant cross-reactivity or interference between Beta-Site APP Cleaving Enzyme 1 (bACE1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Bace1
• Gene Full Name: beta-secretase 1
• Alias Symbols: asp 2;ASP2;aspartyl protease 2;Bace;beta-secretase 1;beta-site amyloid precursor protein cleaving enzyme 1;beta-site APP cleaving enzyme 1;memapsin-2;membrane-associated aspartic protease 2.
• NCBI Gene Id: 29392
• Protein Name: Beta-secretase 1
• Description of Target: Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase (By similarity).
• Uniprot ID: P56819
• Protein Accession #: NP_062077.1
• Nucleotide Accession #: NM_019204.2
• Protein Size (# AA): 501