Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH01652
Size:96T
Price: $525.00
SKU
OKEH01652
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology B4GALT5 ELISA Kit (Human) (OKEH01652) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human B4GALT5 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated B4GALT5 and incubated. The B4GALT5 found in the sample or standards competes with the biotinylated B4GALT5 for limited binding sites on the immobilized anti-human B4GALT5 antibody. Excess unbound biotinylated B4GALT5 and sample or standard B4GALT5 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated B4GALT5 captured in the well and inversely proportional to the amount of B4GALT5 which was contained in the sample or standard.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 9.6% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-B4GALT5 Microplate96 Wells (12 x 8 Well strips)
B4GALT5 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X B4GALT5-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.067 ng/mL
SpecificityNatural and recombinant Human Beta-1,4-galactosyltransferase 5
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolB4GALT5
Gene Full Namebeta-1,4-galactosyltransferase 5
Alias SymbolsB4Gal-T5;beta-1,4-galactosyltransferase 5;beta-1,4-GalT II;beta-1,4-GalT IV;beta-1,4-GalTase 5;beta-1.4-galactosyltransferase V;beta4-GalT IV;beta4Gal-T5;BETA4-GALT-IV;beta4GalT-V;glucosylceramide beta-1,4-galactosyltransferase;gt-V;lacCer synthase;lactosylceramide synthase;UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5;UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5;UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5.
NCBI Gene Id9334
Protein NameO43286
Description of TargetBeta-1,4-galactosyltransferase 5
Uniprot IDhttps://www.uniprot.org/uniprot/O43286
Protein Accession #NP_004767.1
Nucleotide Accession #NM_004776.3
Protein Size (# AA)388
Molecular Weight45 kDa
Write Your Own Review
You're reviewing:B4GALT5 ELISA Kit (Human) (OKEH01652)
Your Rating
We found other products you might like!