B4GALT1 ELISA Kit (Human) (OKCD02396)

Catalog No: OKCD02396

Size:96WELLS

Human B4GALT1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	82-95%	95-105%	99-105%	81-92%
  EDTA Plasma (n=5)	78-96%	97-105%	96-104%	90-102%
  Heparin Plasma (n=5)	80-94%	89-98%	90-101%	86-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-1,4-Galactosyltransferase 1 (b4GALT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-1,4-Galactosyltransferase 1 (b4GALT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-1,4-Galactosyltransferase 1 (b4GALT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Beta-1,4-Galactosyltransferase 1 (b4GALT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	89-98	94
  EDTA Plasma (n=5)	80-103	91
  Heparin Plasma(n=5)	87-96	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-1,4-Galactosyltransferase 1 (b4GALT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-1,4-Galactosyltransferase 1 (b4GALT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-B4GALT1 Microplate: 96 Wells (12 x 8 Well strips)
  • B4GALT1 Lyophilized Standard: 2
  • 100X Biotinylated B4GALT1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.59 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Beta-1,4-Galactosyltransferase 1 (b4GALT1). No significant cross-reactivity or interference between Beta-1,4-Galactosyltransferase 1 (b4GALT1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: B4GALT1
• Gene Full Name: beta-1,4-galactosyltransferase 1
• Alias Symbols: B4GAL-T1;beta-1,4-galactosyltransferase 1;beta-1,4-GalTase 1;beta4Gal-T1;beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase;beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase;CDG2D;GGTB2;glycoprotein-4-beta-galactosyltransferase 2;GT1;GTB;lactose synthase A protein;N-acetyllactosamine synthase;nal synthase;neolactotriaosylceramide beta-1,4-galactosyltransferase;UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1;UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1;UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1.
• NCBI Gene Id: 2683
• Protein Name: Beta-1,4-galactosyltransferase 1
• Description of Target: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
• Uniprot ID: P15291
• Protein Accession #: NP_001488.2
• Nucleotide Accession #: NM_001497.3
• Protein Size (# AA): 398