Azurocidin ELISA Kit (Human) (OKBB00518)

Catalog No: OKBB00518

Size:96 Wells

Human Azurocidin ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human Azurocidin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Azurocidin has been precoated onto 96-well plates. Standards(NS0, I27-P248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Azurocidin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Azurocidin amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	675	1847	2816	873	2020	3174
  SD	28.35	94.2	149.2	48.9	129.3	238.1
  CV%	4.2	5.1	5.3	5.6	6.4	7.5

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human Azurocidin Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human Azurocidin Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human Azurocidin Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human Azurocidin
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: I27-P248
• Description: For quantitative detection of human Azurocidin in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: AZU1
• Gene Full Name: azurocidin 1
• Alias Symbols: Azu 1, AZU1, Azurocidin, CAP37, CAP7_HUMAN, Cationic antimicrobial protein 37, Cationic antimicrobial protein CAP37, HBP, Heparin binding protein, Heparin-binding protein, HUMAZUR, Neutrophil azurocidin
• NCBI Gene Id: 566
• Protein Name: Azurocidin
• Description of Target: Azurocidin, also known as cationic antimicrobial protein CAP37 or heparin-binding protein (HBP), is a protein that in humans is encoded by the AZU1 gene. This encoded protein is a member of the serine protease gene family, but it is not a serine proteinase, because the active site serine and histidine residues are replaced. Azurocidin is mapped to 19p13.3. The protein encoded by this gene is an azurophil granule antibiotic protein, with antibacterial activity. It is also an important multifunctional inflammatory mediator. In addition to it, Azurocidin is also a specific chemoattractant for monocytes. It lacks the chemotactic activity for neutrophils and lymphocytes, and this gene is probably responsible for the wave of monocytes that follows the initial wave of PMNs typical of the inflammatory response.
• Uniprot ID: P20160
• Protein Accession #: NP_001691.1
• Nucleotide Accession #: NM_001700.4