Catalog No: OKEH03486
Size:96T
Price: $525.00
SKU
OKEH03486
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AZGP1 ELISA Kit (Mouse) (OKEH03486) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Azgp1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Azgp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Azgp1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.4% (n = 20)
Mean Inter-assay CV%: <= 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
Azgp1 Microplate96 Wells (12 x 8 Well strips)
Azgp1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Azgp1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39.1 pg/mL
SpecificityNatural and recombinant Mouse Zinc-alpha-2-glycoprotein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAzgp1
Gene Full Namealpha-2-glycoprotein 1, zinc
Alias Symbolsmajor histocompatibility complex class I-like protein;Za;Zag;zinc-alpha-2-glycoprotein;zn-alpha-2-GP.
NCBI Gene Id12007
Protein NameQ64726
Description of TargetZinc-alpha-2-glycoprotein
Uniprot IDhttps://www.uniprot.org/uniprot/Q64726
Protein Accession #NP_038506.2
Nucleotide Accession #NM_013478.2
Protein Size (# AA)307
Molecular Weight35 kDa
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