AXL ELISA Kit (Human) : 96 Wells (OKCA00131)

Catalog No: OKCA00131

Size:96 Wells

Human AXL ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Serum (n=4)	1:1	93%	84-95%
  Serum (n=4)	1:2	92%	85-99%
  Serum (n=4)	1:4	90%	86-95%
  Serum (n=4)	1:8	95%	85-100%

• ELISA Kit Principle: Aviva Systems Biology AXL ELISA Kit (Human) (OKCA00131) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for human AXL has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for human AXL is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample human AXL captured in well.
• ELISA Kit Range: 31.25 pg/mL - 2,000 pg/mL
• ELISA Kit Recovery:

  Sample 1
  Sample Type	Average Recovery	Range
  Serum (n=5)	94%	91-99%

  Sample 2
  Sample Type	Average Recovery	Range
  EDTA Plasma (n=4)	95%	87-99%

• ELISA Kit Reproducibility: Intra-assay: CV% < 8% (n = 20). Inter-assay: CV% < 10% (n = 20)
• ELISA Kit Component:
  • Anti-AXL Microplate: 96 Well (12 x 8 Well Strips)
  • AXL Standard (Lyophilized): 2 x 2 ng
  • 100X AXL Detection Antibody: 1 x 120 uL
  • 100X HRP-Avidin: 1 x 120 uL
  • Detection Antibody Diluent: 1 x 15 mL
  • HRP-Avidin Diluent: 1 x 15 mL
  • Sample Diluent: 1 x 50 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates
• Sensitivity: < 7.81 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of human AXL. No significant cross-reactivity or interference between human AXL and analogues was observed.
  
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: AXL
• Gene Full Name: AXL receptor tyrosine kinase
• Alias Symbols: ARK, UFO, JTK11, Tyro7
• NCBI Gene Id: 558
• Protein Name: Tyrosine-protein kinase receptor UFO
• Description of Target: The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene.
• Uniprot ID: P30530
• Protein Accession #: NP_001265528
• Nucleotide Accession #: NM_001278599
• Protein Size (# AA): 894
• Molecular Weight: 98 kDa