ATP7B ELISA Kit (Human) (OKCD00503)

Catalog No: OKCD00503 (Formerly GWB-KBBST9)

Size:96WELLS

Human ATP7B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Cu++ Transporting Beta Polypeptide (ATP7b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, Cu++ Transporting Beta Polypeptide (ATP7b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Cu++ Transporting Beta Polypeptide (ATP7b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ATP7B Microplate: 96 Wells (12 x 8 Well Strips)
  • ATP7B Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated ATP7B Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.056 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATPase, Cu++ Transporting Beta Polypeptide (ATP7b). No significant cross-reactivity or interference between ATPase, Cu++ Transporting Beta Polypeptide (ATP7b) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ATP7B
• Gene Full Name: ATPase copper transporting beta
• Alias Symbols: ATPase, Cu(2+)- transporting, beta polypeptide;ATPase, Cu++ transporting, beta polypeptide;copper pump 2;copper-transporting ATPase 2;copper-transporting protein ATP7B;PWD;WC1;WD;Wilson disease-associated protein;WND.
• NCBI Gene Id: 540
• Protein Name: Copper-transporting ATPase 2
• Description of Target: Copper ion transmembrane transporter involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile.
• Uniprot ID: P35670
• Protein Accession #: NP_000044
• Nucleotide Accession #: NM_000053.3
• Protein Size (# AA): 1465