ATP5J ELISA Kit (Human) (OKEH03122)

Catalog No: OKEH03122

Size:96T

Human ATP5J ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ATP5J ELISA Kit (Human) (OKEH03122) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ATP5J antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ATP5J and incubated. The ATP5J found in the sample or standards competes with the biotinylated ATP5J for limited binding sites on the immobilized anti-human ATP5J antibody. Excess unbound biotinylated ATP5J and sample or standard ATP5J is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ATP5J captured in the well and inversely proportional to the amount of ATP5J which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.2% (n = 20)
  Mean Inter-assay CV%: <= 7.5% (n = 20)
• ELISA Kit Component:
  • Anti-ATP5J Microplate: 96 Wells (12 x 8 Well strips)
  • ATP5J Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X ATP5J-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.159 ng/mL
• Specificity: Natural and recombinant Human ATP synthase-coupling factor 6, mitochondrial
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: ATP5PF
• Gene Full Name: ATP synthase peripheral stalk subunit F6
• Alias Symbols: ATP synthase subunit h;ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6;ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6;ATP synthase-coupling factor 6, mitochondrial;ATP5;ATP5A;ATP5J;ATPase subunit F6;ATPM;CF6;coupling factor 6;F6;mitochondrial ATP synthase, coupling factor 6;mitochondrial ATP synthase, subunit F6;mitochondrial ATPase coupling factor 6;proliferation-inducing protein 36.
• NCBI Gene Id: 522
• Protein Name: P18859
• Description of Target: ATP synthase-coupling factor 6, mitochondrial
• Uniprot ID: https://www.uniprot.org/uniprot/P18859
• Protein Accession #: NP_001003696.1
• Nucleotide Accession #: NM_001003696.1
• Protein Size (# AA): 108
• Molecular Weight: 13 kDa