ATP5J ELISA Kit (Human) (OKCD00638)

Catalog No: OKCD00638 (Formerly GWB-KBBIS5)

Size:96WELLS

Human ATP5J ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	98-105%	83-95%	92-99%	84-95%
  EDTA plasma(n=5)	91-101%	87-94%	91-102%	94-101%
  heparin plasma(n=5)	95-102%	94-101%	85-105%	92-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coupling Factor 6 (CF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coupling Factor 6 (CF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coupling Factor 6 (CF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Coupling Factor 6 (CF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Matrix	Recovery range (%)	Average(%)
  serum(n=5)	94-105	98
  EDTA plasma(n=5)	80-90	86
  heparin plasma(n=5)	82-91	86

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coupling Factor 6 (CF6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coupling Factor 6 (CF6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ATP5J Microplate: 96 Wells (12 x 8 Well Strips)
  • ATP5J Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated ATP5J Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.068 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Coupling Factor 6 (CF6). No significant cross-reactivity or interference between Coupling Factor 6 (CF6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ATP5PF
• Gene Full Name: ATP synthase peripheral stalk subunit F6
• Alias Symbols: ATP synthase subunit h;ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6;ATP synthase, H+ transporting, mitochondrial Fo complex subunit F6;ATP synthase-coupling factor 6, mitochondrial;ATP5;ATP5A;ATP5J;ATPase subunit F6;ATPM;CF6;coupling factor 6;F6;mitochondrial ATP synthase, coupling factor 6;mitochondrial ATP synthase, subunit F6;mitochondrial ATPase coupling factor 6;proliferation-inducing protein 36.
• NCBI Gene Id: 522
• Protein Name: ATP synthase-coupling factor 6, mitochondrial
• Description of Target: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.
• Uniprot ID: P18859
• Protein Accession #: NP_001003696
• Nucleotide Accession #: NM_001003696.1
• Protein Size (# AA): 108