Catalog No: OKEH06629
Size:96T
Price: $625.00
SKU
OKEH06629
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Ppargc1a ELISA Kit (Pig) (OKEH06629) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ppargc1a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ppargc1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ppargc1a captured in the well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
Ppargc1a Microplate96 Wells (12 x 8 Well strips)
Ppargc1a Lyophilized Standard2 x 10 ng
100X Ppargc1a HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.048 ng/mL
SpecificityNatural and recombinant Pig Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPPARGC1A
Gene Full NamePPARG coactivator 1 alpha
Alias Symbolsperoxisome proliferator activated receptor gamma coactivator 1;peroxisome proliferator activated receptor gamma, coactivator 1 alpha;peroxisome proliferator-activated receptor gamma coactivator 1-alpha;PGC1;PGC1A;PGC-1-alpha;PPAR gamma coactivator 1;PPAR-gamma coactivator 1-alpha;PPARGC1;PPARGC-1;PPARGC-1-alpha.
NCBI Gene Id397013
Protein NameQ865B6
Description of TargetPeroxisome proliferator-activated receptor gamma coactivator 1-alpha
Uniprot IDhttps://www.uniprot.org/uniprot/Q865B6
Protein Accession #NP_999128
Nucleotide Accession #NM_213963
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