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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for ATG4A Antibody (OAAF03545) |
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Predicted Species Reactivity | Human|Mouse |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human ATG4A. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | ATG4A antibody detects endogenous levels of total ATG4A protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:20000 |
Reference | Marino G., J. Biol. Chem. 278:3671-3678(2003). Kabeya Y., J. Cell Sci. 117:2805-2812(2004). Ross M.T., Nature 434:325-337(2005 |
Gene Symbol | ATG4A |
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Gene Full Name | autophagy related 4A cysteine peptidase |
Alias Symbols | APG4 autophagy 4 homolog A;APG4A;ATG4 autophagy related 4 homolog A;AUTL2;AUT-like 2 cysteine endopeptidase;AUT-like 2, cysteine endopeptidase;autophagin 2;Autophagin-2;autophagy-related cysteine endopeptidase 2;autophagy-related protein 4 homolog A;cysteine protease ATG4A;hAPG4A. |
NCBI Gene Id | 115201 |
Protein Name | Cysteine protease ATG4A |
Description of Target | Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms. |
Uniprot ID | Q8WYN0 |
Protein Accession # | NP_443168.2 |
Molecular Weight | 45 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ATG4A Antibody (OAAF03545)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse".
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How long will it take to receive "ATG4A Antibody (OAAF03545)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "ATG4A Antibody (OAAF03545)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ATG4A Antibody (OAAF03545)"?
This target may also be called "APG4 autophagy 4 homolog A;APG4A;ATG4 autophagy related 4 homolog A;AUTL2;AUT-like 2 cysteine endopeptidase;AUT-like 2, cysteine endopeptidase;autophagin 2;Autophagin-2;autophagy-related cysteine endopeptidase 2;autophagy-related protein 4 homolog A;cysteine protease ATG4A;hAPG4A." in publications.
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What is the shipping cost for "ATG4A Antibody (OAAF03545)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ATG4A Antibody (OAAF03545)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ATG4A Antibody (OAAF03545)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "45 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ATG4A Antibody (OAAF03545)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ATG4A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ATG4A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ATG4A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ATG4A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ATG4A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ATG4A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.