ATF4 ELISA Kit (Human) (OKDD00138)

Catalog No: OKDD00138 (Formerly GWB-KBBGB6)

Size:96 Wells

Human ATF4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology ATF4 ELISA Kit (Human) (OKDD00138) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for ATF4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATF4 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ATF4 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATF4 were tested 20 times on one plate, respectively.

  Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATF4 were tested on 3 different plates, 8 replicates in each plate.

  Intra-Assay: CV<10%

  Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-ATF4 Microplate: 96 Wells (12 x 8 Well Strips)
  • ATF4 Lyophlized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X ATF4 Biotinylated Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 ÂuL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 10X Sample Diluent: 1 x 30 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates
• Sensitivity: < 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATF4. No significant cross-reactivity or interference between ATF4 and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ATF4
• Gene Full Name: activating transcription factor 4
• Alias Symbols: Activating transcription factor 4;cAMP response element-binding protein 2;cAMP-dependent transcription factor ATF-4;cAMP-responsive element-binding protein 2;CREB2;CREB-2;cyclic AMP-dependent transcription factor ATF-4;cyclic AMP-responsive element-binding protein 2;DNA-binding protein TAXREB67;TAXREB67;tax-responsive enhancer element B67;tax-responsive enhancer element-binding protein 67;TXREB.
• NCBI Gene Id: 468
• Protein Name: Cyclic AMP-dependent transcription factor ATF-4
• Description of Target: Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production (By similarity). It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. Regulates the induction of DDIT3/CHOP and asparagine synthetase (ASNS) in response to endoplasmic reticulum (ER) stress. In concert with DDIT3/CHOP, activates the transcription of TRIB3 and promotes ER stress-induced neuronal apoptosis by regulating the transcriptional induction of BBC3/PUMA. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. During ER stress response, activates the transcription of NLRP1, possibly in concert with other factors (PubMed:26086088).
• Uniprot ID: P18848
• Protein Accession #: NP_001666.2
• Nucleotide Accession #: NM_001675.4
• Protein Size (# AA): 351
• Molecular Weight: 39 kDa