Aspergillus fumigatus IgM ELISA Kit (Human) (OKNA00104)

Catalog No: OKNA00104

Size:96 Wells

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva Systems Biology Aspergillus fumigatus IgM ELISA Kit (Human) (OKNA00104) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Aspergillus fumigatus antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for Human IgM is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is qualitatively proportional to the amount of sample anti-Aspergillus fumigatus IgM captured the in well.
• ELISA Kit Reproducibility:

  Metric	Sample Type	n=	Mean	CV%
  Intra-Assay	Serum #1	23	0.627	3.1
  Intra-Assay	Serum #2	24	1206	1.8
  Intra-Assay	Serum #3	24	0.845	5.1
  Inter-Assay	Serum #1	12	33.03	6.7
  Inter-Assay	Serum #2	12	45.24	3.8
  Inter-Assay	Serum #3	12	22.71	8.9

• ELISA Kit Component:
  • Aspergillus fumigatus IgM Well Plate: 96 Wells (12 x 8 Well Strips)
  • IgM Sample Diluent: 1 x 100 mL
  • Stop Solution: 1 x 15 mL
  • 20X Wash Solution: 1 x 50 mL
  • Anti-IgM HRP Conjugate: 1 x 20 mL
  • TMB Substrate: 1 x 15 mL
  • Aspergillus fumigatus Positive Control: 1 x 2 mL
  • Aspergillus fumigatus Cut-Off Control: 1 x 3 mL
  • Aspergillus fumigatus Negative Control: 1 x 2 mL
• Additional Information: Specificity is determined as the probability of the assay indicating a negative score in samples absent of the specific analyte.
  Sensitivity is determined as the probability of the assay indicating a positive score in samples with the specific analyte present.
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma
• Assay Info: Assay Methodology: Quantitative Reverse Capture Sandwich ELISA

Target Info

• Alias Symbols: AF
• Protein Name: Aspergillus fumigatus IgM
• Description of Target: Aspergillus fumigatus is a fungus of the genus Aspergillus, and is one of the most common Aspergillus species to cause disease in individuals with an immunodeficiency. A. fumigatus, a saprotroph widespread in nature, is typically found in soil and decaying organic matter. Colonies of the fungus produce thousands of small conidia (2-3 μm) that readily become airborne. The fungus is capable of growth at temperatures up to 50 C, with conidia surviving at 70 C. Humans are continuously in contact with these asexual spores and it is estimated that an individual inhales several hundred conidia each day; typically these are quickly eliminated by the immune system. Aspergillosis develops mainly in individuals who are immunocompromised, either from disease or from immunosuppressive drugs, and is a leading cause of death in acute leukemia and hematopoietic stem cell transplantation. The term aspergillosis comprises a number of different diseases caused by fungi of the genus Aspergillus. The most common forms are allergic bronchopulmonary aspergillosis (ABPA), pulmonary aspergilloma and invasive aspergillosis (IA). Allergic bronchopulmonary aspergillosis is characterized by an exaggerated response of the immune system to Aspergillus species. It occurs in patients suffering from asthma or cystic fibrosis. Clinically, ABPA manifests as a bronchial asthma with transient pulmonary infiltrates that may proceed to proximal bronchiectasis and lung fibrosis. Aspergilloma, commonly referred to as “fungus ball,” occurs in preexisting pulmonary cavities that were caused by tuberculosis, sarcoidosis, or other bullous lung disorders. The fungus settles in a cavity and grows until it forms a compact sphere, which incorporates dead tissue from the surrounding lung, mucus, and other debris. Patients are usually asymptomatic and often coexist for decades with aspergillomae prior to incidental diagnosis. However, it may cause hemoptysis. Invasive aspergillosis is a rapidly progressive, often fatal disease, targeting severely immunocompromised patients, including those with hematological malignancies such as leukemia, those who have received solid organ or hematopoietic stem cell transplants, and individuals with chronic granulomatous disease or advanced AIDS. IA is characterized by invasion of blood vessels, resulting in multifocal infiltrates. Dissemination to other organs, particularly the central nervous system, may occur.