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Catalog No: OKCD00920 (Formerly GWB-KBBPT3)
Size:96WELLS
Price: $700.00
SKU
OKCD00920
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Datasheets/ManualsPrintable datasheet for ASNS ELISA Kit (Mouse) (OKCD00920)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)85-95%83-97%96-103%79-88%
EDTA plasma(n=5)94-105%90-99%95-105%89-104%
heparin plasma(n=5)93-101%84-98%80-101%88-96%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Asparagine Synthetase (ASNS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Asparagine Synthetase (ASNS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Asparagine Synthetase (ASNS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Asparagine Synthetase (ASNS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)87-9490
EDTA plasma(n=5)79-10391
heparin plasma(n=5)91-9994
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Asparagine Synthetase (ASNS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Asparagine Synthetase (ASNS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Asns Microplate96 Wells (12 x 8 Well Strips)
Asns Lyophilized Standard2 x 20 ng
100X Biotinylated Asns Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 0.108 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Asparagine Synthetase (ASNS). No significant cross-reactivity or interference between Asparagine Synthetase (ASNS) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolAsns
Gene Full Nameasparagine synthetase
Alias Symbolsasparagine synthetase [glutamine-hydrolyzing];glutamine-dependent asparagine synthetase.
NCBI Gene Id27053
Protein NameAsparagine synthetase [glutamine-hydrolyzing]
Description of TargetAsparagine synthetase [glutamine-hydrolyzing]
Uniprot IDQ61024
Protein Accession #NP_001171546
Nucleotide Accession #NM_001178075.1
Protein Size (# AA)561
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