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Catalog No: OKCD00572 (Formerly GWB-KBBXP7)
Size:96WELLS
Price: $675.00
SKU
OKCD00572
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Datasheets/ManualsPrintable datasheet for ARPC2 ELISA Kit (Human) (OKCD00572)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Related Protein 2/3 Complex Subunit 2 (ARPC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Related Protein 2/3 Complex Subunit 2 (ARPC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Related Protein 2/3 Complex Subunit 2 (ARPC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ARPC2 Microplate96 Wells (12 x 8 Well Strips)
ARPC2 Lyophilized Standard2 x 10 ng
100X Biotinylated ARPC2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.068 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Actin Related Protein 2/3 Complex Subunit 2 (ARPC2). No significant cross-reactivity or interference between Actin Related Protein 2/3 Complex Subunit 2 (ARPC2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolARPC2
Gene Full Nameactin related protein 2/3 complex subunit 2
Alias Symbolsactin related protein 2/3 complex, subunit 2, 34kDa;actin-related protein 2/3 complex subunit 2;ARC34;arp2/3 complex 34 kDa subunit;ARP2/3 protein complex subunit 34;p34-Arc;PNAS-139;PRO2446;testis tissue sperm-binding protein Li 53e.
NCBI Gene Id10109
Protein NameActin-related protein 2/3 complex subunit 2
Description of TargetFunctions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
Uniprot IDO15144
Protein Accession #NP_005722
Nucleotide Accession #NM_005731.3
Protein Size (# AA)300
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