Catalog No: OKCD01233 (Formerly GWB-KBBXT5)
Size:96WELLS
Price: $750.00
SKU
OKCD01233
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for ARL15 ELISA Kit (Human) (OKCD01233) |
---|
Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADP Ribosylation Factor Like Protein 15 (ARL15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ADP Ribosylation Factor Like Protein 15 (ARL15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADP Ribosylation Factor Like Protein 15 (ARL15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ADP Ribosylation Factor Like Protein 15 (ARL15) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 78-5,000pg/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADP Ribosylation Factor Like Protein 15 (ARL15) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADP Ribosylation Factor Like Protein 15 (ARL15) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
| ||||||||||||||||||||||
Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 29 pg/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of ADP Ribosylation Factor Like Protein 15 (ARL15). No significant cross-reactivity or interference between ADP Ribosylation Factor Like Protein 15 (ARL15) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | ARL15 |
---|---|
Gene Full Name | ADP ribosylation factor like GTPase 15 |
Alias Symbols | ADP-ribosylation factor-like 15;ADP-ribosylation factor-like protein 15;ADP-ribosylation factor-related protein 2;ARF-related protein 2;ARFRP2. |
NCBI Gene Id | 54622 |
Protein Name | ADP-ribosylation factor-like protein 15 |
Description of Target | ADP-ribosylation factor-like protein 15 |
Uniprot ID | Q9NXU5 |
Protein Accession # | NP_061960 |
Nucleotide Accession # | NM_019087.2 |
Protein Size (# AA) | 204 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
Write Your Own Review