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Catalog No: OKCD01233 (Formerly GWB-KBBXT5)
Size:96WELLS
Price: $750.00
SKU
OKCD01233
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Datasheets/ManualsPrintable datasheet for ARL15 ELISA Kit (Human) (OKCD01233)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADP Ribosylation Factor Like Protein 15 (ARL15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ADP Ribosylation Factor Like Protein 15 (ARL15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADP Ribosylation Factor Like Protein 15 (ARL15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ADP Ribosylation Factor Like Protein 15 (ARL15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADP Ribosylation Factor Like Protein 15 (ARL15) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADP Ribosylation Factor Like Protein 15 (ARL15) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ARL15 Microplate96 Wells (12 x 8 Well Strips)
ARL15 Lyophilized Standard2 x 5 ng
100X Biotinylated ARL15 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 29 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of ADP Ribosylation Factor Like Protein 15 (ARL15). No significant cross-reactivity or interference between ADP Ribosylation Factor Like Protein 15 (ARL15) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolARL15
Gene Full NameADP ribosylation factor like GTPase 15
Alias SymbolsADP-ribosylation factor-like 15;ADP-ribosylation factor-like protein 15;ADP-ribosylation factor-related protein 2;ARF-related protein 2;ARFRP2.
NCBI Gene Id54622
Protein NameADP-ribosylation factor-like protein 15
Description of TargetADP-ribosylation factor-like protein 15
Uniprot IDQ9NXU5
Protein Accession #NP_061960
Nucleotide Accession #NM_019087.2
Protein Size (# AA)204
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