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Catalog No: OKEH02367
Size:96T
Price: $625.00
SKU
OKEH02367
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ARID4B ELISA Kit (Human) (OKEH02367) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ARID4B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ARID4B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ARID4B captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.2% (n = 20)
Mean Inter-assay CV%: < 8.6% (n = 20)
ELISA Kit Component
ComponentAmount
ARID4B Microplate96 Wells (12 x 8 Well strips)
ARID4B Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ARID4B Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.23 ng/mL
SpecificityNatural and recombinant Human AT-rich interactive domain-containing protein 4B
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolARID4B
Gene Full NameAT-rich interaction domain 4B
Alias Symbols180 kDa Sin3-associated polypeptide;ARID domain-containing protein 4B;AT rich interactive domain 4B (RBP1-like);AT-rich interactive domain-containing protein 4B;BCAA;BRCAA1;breast cancer-associated antigen 1;breast cancer-associated antigen BRCAA1;breast carcinoma-associated antigen;histone deacetylase complex subunit SAP180;Rb-binding protein homolog;RBBP1L1;RBP1L1;retinoblastoma-binding protein 1-like 1;SAP180;SIN3A-associated protein 180;sin3-associated polypeptide p180.
NCBI Gene Id51742
Protein NameQ4LE39
Description of TargetAT-rich interactive domain-containing protein 4B
Uniprot IDhttps://www.uniprot.org/uniprot/Q4LE39
Protein Accession #NP_001193723.1
Nucleotide Accession #NM_001206794.1
Protein Size (# AA)1312
Molecular Weight148 kDa
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