Catalog No: OKEH04455
Size:96T
Price: $525.00
SKU
OKEH04455
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Arg1 ELISA Kit (Rat) (OKEH04455) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Arg1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Arg1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Arg1 captured in the well.
ELISA Kit Range2.5-160ng/mL
ELISA Kit Component
ComponentAmount
Anti-Arg1 Microplate96 Wells (12 x 8 Well Strips)
Arg1 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Arg1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity1.25 ng/mL
SpecificityNatural and recombinant Rat Arginase-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolArg1
Gene Full Namearginase 1
Alias SymbolsAI type I arginase;arginase, liver;arginase-1;liver-type arginase;type I arginase.
NCBI Gene Id29221
Protein NameP07824
Description of TargetArginase-1
Uniprot IDhttps://www.uniprot.org/uniprot/P07824
Protein Accession #NP_058830.2
Nucleotide Accession #NM_017134.3
Protein Size (# AA)323
Molecular Weight35 KDa
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