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Catalog No: OKEH03009
Size:96T
Price: $475.00
SKU
OKEH03009
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology APP ELISA Kit (Mouse) (OKEH03009) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for App has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for App is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample App captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 112%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.3% (n = 20)
Mean Inter-assay CV%: <= 8.7% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-App Microplate96 Wells (12 x 8 Well strips)
App Lyophilized Standard2
100X Biotinylated App Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity39 pg/mL
SpecificityNatural and recombinant Mouse Amyloid beta A4 protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolApp
Gene Full Nameamyloid beta (A4) precursor protein
Alias SymbolsA;Abe;Abeta;Abpp;Adap;Ag;alzheimer disease amyloid A4 protein homolog;amyloid beta A4 protein;amyloid precursor protein;amyloid-beta A4 protein;amyloid-beta precursor protein;amyloidogenic glycoprotein;appican;bet;beta-amyloid precursor protein;betaApp;C;Cvap;E030013M08Rik;protease nexin II.
NCBI Gene Id11820
Protein NameP12023
Description of TargetAmyloid-beta A4 protein
Uniprot IDhttps://www.uniprot.org/uniprot/P12023
Protein Accession #NP_001185752.1
Nucleotide Accession #NM_001198823.1
Protein Size (# AA)770
Molecular Weight87 kDa
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