APP ELISA Kit (Human) : 96 Wells (OKEH02647)

Catalog No: OKEH02647

Size:96T

Human APP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology APP ELISA Kit (Human) (OKEH02647) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for APP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for APP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample APP captured in well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.3% (n = 20)
  Mean Inter-assay CV%: < 10.1% (n = 20)
• ELISA Kit Component:
  • Anti-APP Microplate: 96 Wells (12 x 8 Well strips)
  • APP Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated APP Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 10 pg/mL
• Specificity: Natural and recombinant Human Amyloid beta A4 protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: APP
• Gene Full Name: amyloid beta precursor protein
• Alias Symbols: AAA;ABETA;ABPP;AD1;alpha-sAPP;alzheimer disease amyloid protein;amyloid beta (A4) precursor protein;amyloid beta A4 protein;amyloid precursor protein;amyloid-beta precursor protein;APPI;beta-amyloid peptide;beta-amyloid peptide(1-40);beta-amyloid peptide(1-42);beta-amyloid precursor protein;cerebral vascular amyloid peptide;CTFgamma;CVAP;peptidase nexin-II;PN2;PN-II;preA4;protease nexin-II;testicular tissue protein Li 2.
• NCBI Gene Id: 351
• Protein Name: P05067
• Description of Target: Amyloid-beta A4 protein
• Uniprot ID: https://www.uniprot.org/uniprot/P05067
• Protein Accession #: NP_000475.1
• Nucleotide Accession #: NM_000484.3
• Protein Size (# AA): 770
• Molecular Weight: 87 kDa