APOL1 High Sensitivity ELISA Kit (Human) (OKCD01378)

Catalog No: OKCD01378

Size:96WELLS

Human APOL1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	91-105%	89-104%	80-92%	92-102%
  EDTA Plasma (n=5)	89-101%	96-104%	98-105%	87-94%
  Heparin Plasma (n=5)	87-101%	81-98%	78-101%	91-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Apolipoprotein L (APOL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Apolipoprotein L (APOL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Apolipoprotein L (APOL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Apolipoprotein L (APOL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	94-101	97
  EDTA Plasma (n=5)	86-94	90
  Heparin Plasma (n=5)	84-91	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Apolipoprotein L (APOL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Apolipoprotein L (APOL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-APOL1 Microplate: 96 Wells (12 x 8 Well Strips)
  • APOL1 Lyophilized Standard: 2
  • 100X Biotinylated APOL1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.112 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Apolipoprotein L (APOL1). No significant cross-reactivity or interference between Apolipoprotein L (APOL1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: APOL1
• Gene Full Name: apolipoprotein L1
• Alias Symbols: APOL;APO-L;APOL-I;Apolipoprotein L;apolipoprotein L 1;apolipoprotein L1;apolipoprotein L1-B3;Apolipoprotein L-I;FSGS4.
• NCBI Gene Id: 8542
• Protein Name: Apolipoprotein L1
• Description of Target: May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.
• Uniprot ID: O14791
• Protein Accession #: NP_001130012.1
• Nucleotide Accession #: NM_001136540.1