Catalog No: OKEH03198
Size:96T
Price: $525.00
SKU
OKEH03198
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH03198
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology APOE ELISA Kit (Rat) (OKEH03198) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apoe has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Apoe is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apoe captured in well.
ELISA Kit Range7.8-500ng/mL
ELISA Kit Component
ComponentAmount
Anti-Apoe Microplate96 Wells (12 x 8 Well strips)
Apoe Lyophilized Standard2
100X Biotinylated Apoe Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity3.97 ng/mL
SpecificityNatural and recombinant Rat Apolipoprotein E
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolApoe
Gene Full Nameapolipoprotein E
Alias Symbolsapo-E;APOEA;apolipoprotein E.
NCBI Gene Id25728
Protein NameP02650
Description of TargetApolipoprotein E
Uniprot IDhttps://www.uniprot.org/uniprot/P02650
Protein Accession #NP_001257610.1
Nucleotide Accession #NM_001270681.1
Protein Size (# AA)312
Molecular Weight36 kDa
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