APOE ELISA Kit (Mouse) : 96 Wells (OKCA00134)

Catalog No: OKCA00134

Size:96 Wells

Mouse APOE ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Serum (n=4)	1:100	99%	96-101%
  Serum (n=4)	1:200	85%	81-89%
  Serum (n=4)	1:400	100%	97-103%
  Serum (n=4)	1:800	83%	80-86%

• ELISA Kit Principle: Aviva Systems Biology APOE ELISA Kit (Mouse) (OKCA00134) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Mouse APOE and blocked. Standards or samples are added to the plate wells along with a horseradish peroxidase (HRP) conjugated antibody specific to APOE and incubated. APOE immobilized in the wells competes with the liquid phase APOE contained in the samples or standards for binding with the HRP-Conjugated APOE Antibody. Wells are washed removing unbound APOE, unbound HRP-Conjugated APOE Antibody or APOE/HRP-Conjugated APOE Antibody complex. HRP-Conjugated APOE Antibody bound to APOE immobilized in the well remains. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of HRP-Conjugated APOE Antibody captured in the well and inversely proportional to the amount of APOE which was contained in the sample or standard.
• ELISA Kit Range: 6.25 ng/mL - 400 ng/mL
• ELISA Kit Recovery:

  Sample 1
  Sample Type	Average Recovery	Range
  Serum (n=5)	97%	93-100%

  Sample 2
  Sample Type	Average Recovery	Range
  EDTA Plasma (n=4)	99%	95-104%

• ELISA Kit Reproducibility: Intra-assay: CV% < 8% (n = 20). Inter-assay: CV% < 10% (n = 20)
• ELISA Kit Component:
  • APOE Microplate: 96 Well (12 x 8 Well Strips)
  • APOE Standard (Lyophilized): 2 x 400 ng
  • 100X HRP-Conjugate: 1 x 60 uL
  • HRP-Conjugate Diluent: 1 x 10 mL
  • Sample Diluent: 2 x 20 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Culture Supernatants, Tissue homogenates
• Sensitivity: < 1.56 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of
  mouse Apo-E. No significant cross-reactivity or interference between mouse Apo-E and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Competitive Immunoassay

Target Info

• Gene Symbol: APOE
• Gene Full Name: apolipoprotein E
• Alias Symbols: A, Apo-E, AI255918
• NCBI Gene Id: 11816
• Protein Name: Apolipoprotein E
• Description of Target: This gene encodes a member of the apolipoprotein A1/A4/E family of proteins. This protein is involved in the transport of lipoproteins in the blood. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. Homozygous knockout mice for this gene accumulate high levels of cholesterol in the blood and develop atherosclerosis. Different alleles of this gene have been associated with either increased risk or a protective effect for Alzheimer's disease in human patients. This gene maps to chromosome 7 in a cluster with the related apolipoprotein C1, C2 and C4 genes.
• Uniprot ID: P08226
• Protein Accession #: NP_001292748
• Nucleotide Accession #: NM_001305819
• Protein Size (# AA): 311
• Molecular Weight: 36 kDa