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Datasheets/Manuals | Printable datasheet for OAAB09815 |
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Predicted Species Reactivity | Human |
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Product Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Clonality | Polyclonal |
Isotype | Ig |
Host | Rabbit |
Application | WB, IHC |
Additional Information | Precautions: APOBEC3G (CEM15) Antibody (Center E133) is for research use only and not for use in diagnostic or therapeutic procedures. |
:: | Function: DNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV-1 infection) by triggering G-to-A hypermutation in the newly synthesized viral DNA. The replacements C-to-U in the minus strand DNA of HIV-1 during reverse transcription, leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF), that prevents the incorporation of APOBEC3G into progeny HIV-1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome May also prevent the transposition of a subset of retroelements Binds a variety of RNAs, but does not display detectable APOB, NF1 and NAT1 mRNA editing |
:: | Cellular Location: Cytoplasm. Nucleus. Note=Mainly cytoplasmic. Small amount are found in the nucleus. During HIV-1 infection, virion-encapsidated in absence of HIV-1 VIF Tissue Location: Expressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. Also expressed in non-permissive peripheral blood mononuclear cells, and several tumor cell lines; no expression detected in permissive lymphoid and non-lymphoid cell lines |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This APOBEC3G (CEM15) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-148 amino acids from the Central region of human APOBEC3G (CEM15). |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Application Info | WB: 1:1000 IHC-P: 1:50~100 |
Gene Symbol | APOBEC3G |
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Alias Symbols | A3G, ARCD, ARP9, ARP-9, CEM15, CEM-15, MDS019, bK150C2.7, dJ494G10.1 |
NCBI Gene Id | 60489 |
Protein Name | DNA dC->dU-editing enzyme APOBEC-3G |
Description of Target | Purified Rabbit Polyclonal Antibody (Pab) |
Uniprot ID | Q9HC16 |
Protein Accession # | NP_001006667.1 |
Molecular Weight | 46408 Da |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "APOBEC3G Antibody - middle region (Phospho-E133) (OAAB09815)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "APOBEC3G Antibody - middle region (Phospho-E133) (OAAB09815)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "APOBEC3G Antibody - middle region (Phospho-E133) (OAAB09815)" provided in?
This item is provided in "Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "APOBEC3G Antibody - middle region (Phospho-E133) (OAAB09815)"?
This target may also be called "A3G, ARCD, ARP9, ARP-9, CEM15, CEM-15, MDS019, bK150C2.7, dJ494G10.1" in publications.
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What is the shipping cost for "APOBEC3G Antibody - middle region (Phospho-E133) (OAAB09815)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "APOBEC3G Antibody - middle region (Phospho-E133) (OAAB09815)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "APOBEC3G Antibody - middle region (Phospho-E133) (OAAB09815)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "46408 Da".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "APOBEC3G Antibody - middle region (Phospho-E133) (OAAB09815)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "APOBEC3G"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "APOBEC3G"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "APOBEC3G"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "APOBEC3G"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "APOBEC3G"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "APOBEC3G"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.