APOA2 ELISA Kit (Mouse) (OKCD02376)

Catalog No: OKCD02376

Size:96WELLS

Mouse APOA2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	91-105%	90-98%	83-93%	94-101%
  EDTA Plasma (n=5)	80-91%	78-104%	78-101%	84-92%
  Heparin Plasma (n=5)	91-104%	85-103%	97-105%	83-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A2 (APOA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A2 (APOA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A2 (APOA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Apolipoprotein A2 (APOA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 6.25-400ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	90-97	93
  EDTA Plasma (n=5)	87-97	92
  Heparin Plasma(n=5)	85-94	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein A2 (APOA2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein A2 (APOA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-APOA2 Microplate: 96 Wells (12 x 8 Well strips)
  • APOA2 Lyophilized Standard: 2
  • 100X Biotinylated APOA2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.076 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A2 (APOA2). No significant cross-reactivity or interference between Apolipoprotein A2 (APOA2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Apoa2
• Gene Full Name: apolipoprotein A-II
• Alias Symbols: A;Al;Alp-2;Ap;Apoa-2;ApoAII;ApoA-II;Apo-AII;apolipoprotein A2;apolipoprotein A-II;Hdl-;Hdl-1.
• NCBI Gene Id: 11807
• Protein Name: Apolipoprotein A-II
• Description of Target: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
• Uniprot ID: P09813
• Protein Accession #: NP_001292478.1
• Nucleotide Accession #: NM_001305549.1
• Protein Size (# AA): 102