Catalog No: OKCD01229 (Formerly GWB-KBBBQ6)
Size:96WELLS
Price: $700.00
SKU
OKCD01229
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Datasheets/ManualsPrintable datasheet for APM1 ELISA Kit (Dog) (OKCD01229)
Product Info
Predicted Species ReactivityCanis lupus|Dog
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)80-101%97-105%81-101%96-103%
EDTA plasma(n=5)98-105%80-101%91-101%79-104%
heparin plasma(n=5)78-96%94-101%95-104%96-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin (ADPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adiponectin (ADPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adiponectin (ADPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Adiponectin (ADPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)98-105101
EDTA plasma(n=5)95-10598
heparin plasma(n=5)98-105102
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adiponectin (ADPN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adiponectin (ADPN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-apM1 Microplate96 Wells (12 x 8 Well Strips)
apM1 Lyophilized Standard2 x 5 ng
100X Biotinylated apM1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 33 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Adiponectin (ADP). No significant cross-reactivity or interference between Adiponectin (ADP) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolADIPOQ
Gene Full Nameadiponectin, C1Q and collagen domain containing
Alias SymbolsACDC;adipocyte, C1Q and collagen domain containing;adiponectin;APM1.
NCBI Gene Id403625
Protein NameAdiponectin
Description of TargetAdiponectin
Uniprot IDQ76C76
Protein Accession #NP_001006645
Nucleotide Accession #NM_001006644.1
Protein Size (# AA)244
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