Catalog No: OKEH01349
Size:96T
Price: $525.00
SKU
OKEH01349
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology APLN13 ELISA Kit (Human) (OKEH01349)is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-APLN13 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated APLN13 and incubated. The APLN13 found in the sample or standards competes with the biotinylated APLN13 for limited binding sites on the immobilized anti-APLN13 antibody. Excess unbound biotinylated APLN13 and sample or standard APLN13 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated APLN13 captured in the well and inversely proportional to the amount of APLN13 which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 78-5000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.0% (n = 20) Mean Inter-assay CV%: < 6.5% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
Sensitivity | 33 pg/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Apelin-13 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Gene Symbol | APLN13 |
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Alias Symbols | APEL, XNPEP2 |
NCBI Gene Id | 8862 |
Protein Name | Apelin |
Description of Target | Apelin gene encodes a pre-proprotein of 77 amino acids, with a signal peptide in the N-terminal region. After translocation into the endoplasmic reticulum and cleavage of the signal peptide, the proprotein of 55 amino acids may generate several active fragments: a 36 amino acid peptide corresponding to the sequence 42-77 (apelin 36), a 17 amino acid peptide corresponding to the sequence 61-77 (apelin 17) and a 13 amino acid peptide corresponding to the sequence 65-77 (apelin 13). |
Uniprot ID | Q9ULZ1 |
Protein Accession # | NP_059109 |
Nucleotide Accession # | NM_017413 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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