Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH01349
Size:96T
Price: $525.00
SKU
OKEH01349
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology APLN13 ELISA Kit (Human) (OKEH01349)is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-APLN13 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated APLN13 and incubated. The APLN13 found in the sample or standards competes with the biotinylated APLN13 for limited binding sites on the immobilized anti-APLN13 antibody. Excess unbound biotinylated APLN13 and sample or standard APLN13 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated APLN13 captured in the well and inversely proportional to the amount of APLN13 which was contained in the sample or standard.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.0% (n = 20)
Mean Inter-assay CV%: < 6.5% (n = 20)
ELISA Kit Component
ComponentAmount
APLN13 Microplate96 Wells (12 x 8 Well strips)
APLN13 Lyophilized Standard2
100X APLN13 Biotin-Complex1 x 60 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity33 pg/mL
SpecificityNatural and recombinant Human Apelin-13
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolAPLN13
Alias SymbolsAPEL, XNPEP2
NCBI Gene Id8862
Protein NameApelin
Description of TargetApelin gene encodes a pre-proprotein of 77 amino acids, with a signal peptide in the N-terminal region. After translocation into the endoplasmic reticulum and cleavage of the signal peptide, the proprotein of 55 amino acids may generate several active fragments: a 36 amino acid peptide corresponding to the sequence 42-77 (apelin 36), a 17 amino acid peptide corresponding to the sequence 61-77 (apelin 17) and a 13 amino acid peptide corresponding to the sequence 65-77 (apelin 13).
Uniprot IDQ9ULZ1
Protein Accession #NP_059109
Nucleotide Accession #NM_017413
Write Your Own Review
You're reviewing:APLN13 ELISA Kit (Human) (OKEH01349)
Your Rating