API5 ELISA Kit (Human) (OKCD00933)

Catalog No: OKCD00933 (Formerly GWB-KBBFS5)

Size:96WELLS

Human API5 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  	87-94%	79-90%	91-98%	85-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apoptosis Inhibitor 5 (API5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apoptosis Inhibitor 5 (API5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apoptosis Inhibitor 5 (API5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Apoptosis Inhibitor 5 (API5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  	95-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apoptosis Inhibitor 5 (API5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apoptosis Inhibitor 5 (API5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-API5 Microplate: 96 Wells (12 x 8 Well Strips)
  • API5 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated API5 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.122 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Apoptosis Inhibitor 5 (API5). No significant cross-reactivity or interference between Apoptosis Inhibitor 5 (API5) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: API5
• Gene Full Name: apoptosis inhibitor 5
• Alias Symbols: AAC11;AAC-11;antiapoptosis clone 11 protein;apoptosis inhibitor 5;cell migration-inducing gene 8 protein;Fibroblast growth factor 2-interacting factor;fibroblast growth factor 2-interacting factor 2;FIF;migration-inducing protein MIG8;Protein XAGL.
• NCBI Gene Id: 8539
• Protein Name: Apoptosis inhibitor 5
• Description of Target: Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
• Uniprot ID: Q9BZZ5
• Protein Accession #: NP_001136402
• Nucleotide Accession #: NM_001142930.1
• Protein Size (# AA): 524