APEX1 Antibody (OAGA00005)

Catalog No: OAGA00005

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
OAGA00005 diluted at 1:3000

APE1 antibody [N1], N-term detects APEX1 protein at nucleus on Saos2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Saos2 xenograft.
APE1 antibody [N1], N-term (OAGA00005) dilution: 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using APE1(OAGA00005) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq]
  Positive Controls: A549
  Cellular Localization: Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 1 and 45 of APE1 (Uniprot ID#P27695)
• Purification: Affinity purified by Protein A.
• Concentration: 5.04 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: APEX1
• Gene Full Name: DNA-(apurinic or apyrimidinic site) lyase
• Alias Symbols: APE, APX, APE1, APEN, APEX, HAP1, REF1
• NCBI Gene Id: 328
• Protein Name: DNA-(apurinic or apyrimidinic site) lyase
• Uniprot ID: P27695
• Protein Accession #: NP_001231178.1
• Molecular Weight: 36 kDa