Catalog No: OKEH02227
Size:96T
Price: $525.00
SKU
OKEH02227
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology AP1M1 ELISA Kit (Human) (OKEH02227) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human AP1M1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated AP1M1 and incubated. The AP1M1 found in the sample or standards competes with the biotinylated AP1M1 for limited binding sites on the immobilized anti-Human AP1M1 antibody. Excess unbound biotinylated AP1M1 and sample or standard AP1M1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated AP1M1 captured in the well and inversely proportional to the amount of AP1M1 which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.3% (n = 20) Mean Inter-assay CV%: < 8.1% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.12 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human AP-1 complex subunit mu-1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Gene Symbol | AP1M1 |
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Gene Full Name | adaptor related protein complex 1 subunit mu 1 |
Alias Symbols | adapter-related protein complex 1 subunit mu-1;adaptor protein complex AP-1 mu-1 subunit;adaptor protein complex AP-1 subunit mu-1;adaptor related protein complex 1 mu 1 subunit;Adaptor-related protein complex 1 subunit mu-1;AP-1 complex subunit mu-1;AP47;AP-mu chain family member mu1A;CLAPM2;clathrin adaptor protein AP47;clathrin assembly protein complex 1 medium chain 1;clathrin assembly protein complex 1 mu-1 medium chain 1;clathrin assembly protein complex 1, medium chain;clathrin assembly protein complex AP1, mu subunit;clathrin coat assembly protein AP47;clathrin coat-associated protein AP47;CLTNM;golgi adaptor AP-1 47 kDa protein;golgi adaptor HA1/AP1 adaptin mu-1 subunit;HA1 47 kDa subunit;mu1A;MU-1A;mu1A-adaptin;mu-adaptin 1. |
NCBI Gene Id | 8907 |
Protein Name | Q9BXS5 |
Description of Target | AP-1 complex subunit mu-1 |
Uniprot ID | https://www.uniprot.org/uniprot/Q9BXS5 |
Protein Accession # | NP_001123996.1 |
Nucleotide Accession # | NM_001130524.1 |
Protein Size (# AA) | 423 |
Molecular Weight | 49 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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