Catalog No: OKEH02227
Size:96T
Price: $525.00
SKU
OKEH02227
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AP1M1 ELISA Kit (Human) (OKEH02227) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human AP1M1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated AP1M1 and incubated. The AP1M1 found in the sample or standards competes with the biotinylated AP1M1 for limited binding sites on the immobilized anti-Human AP1M1 antibody. Excess unbound biotinylated AP1M1 and sample or standard AP1M1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated AP1M1 captured in the well and inversely proportional to the amount of AP1M1 which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 8.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-AP1M1 Microplate96 Wells (12 x 8 Well strips)
AP1M1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X AP1M1 Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.12 ng/mL
SpecificityNatural and recombinant Human AP-1 complex subunit mu-1
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolAP1M1
Gene Full Nameadaptor related protein complex 1 subunit mu 1
Alias Symbolsadapter-related protein complex 1 subunit mu-1;adaptor protein complex AP-1 mu-1 subunit;adaptor protein complex AP-1 subunit mu-1;adaptor related protein complex 1 mu 1 subunit;Adaptor-related protein complex 1 subunit mu-1;AP-1 complex subunit mu-1;AP47;AP-mu chain family member mu1A;CLAPM2;clathrin adaptor protein AP47;clathrin assembly protein complex 1 medium chain 1;clathrin assembly protein complex 1 mu-1 medium chain 1;clathrin assembly protein complex 1, medium chain;clathrin assembly protein complex AP1, mu subunit;clathrin coat assembly protein AP47;clathrin coat-associated protein AP47;CLTNM;golgi adaptor AP-1 47 kDa protein;golgi adaptor HA1/AP1 adaptin mu-1 subunit;HA1 47 kDa subunit;mu1A;MU-1A;mu1A-adaptin;mu-adaptin 1.
NCBI Gene Id8907
Protein NameQ9BXS5
Description of TargetAP-1 complex subunit mu-1
Uniprot IDhttps://www.uniprot.org/uniprot/Q9BXS5
Protein Accession #NP_001123996.1
Nucleotide Accession #NM_001130524.1
Protein Size (# AA)423
Molecular Weight49 kDa
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