Catalog No: OKDD00125 (Formerly GWB-KBBXT6)
Size:96 Wells
Price: $800.00
SKU
OKDD00125
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Datasheets/ManualsPrintable datasheet for AOAH ELISA Kit (Human) (OKDD00125)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology AOAH ELISA Kit (Human) (OKDD00125) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for AOAH has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AOAH is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample AOAH captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AOAH were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AOAH were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-AOAH Microplate96 Wells (12 x 8 Well Strips)
AOAH Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X AOAH Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, cell lysates
Sensitivity< 0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of AOAH. No significant cross-reactivity or interference between AOAH and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolAOAH
Gene Full Nameacyloxyacyl hydrolase
Alias Symbolsacyloxyacyl hydrolase;acyloxyacyl hydrolase (neutrophil).
NCBI Gene Id313
Protein NameAcyloxyacyl hydrolase
Description of TargetRemoves the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides (PubMed:1883828, PubMed:8089145, PubMed:29343645). By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses (By similarity). In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria (By similarity).
Uniprot IDP28039
Protein Accession #NP_001170977.1
Nucleotide Accession #NM_001177506.1
Protein Size (# AA)575
Molecular Weight65 kDa
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