ANXA2 ELISA kit (Rat) (OKCD02307)

Catalog No: OKCD02307

Size:96WELLS

Rat ANXA2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	86-97%	86-93%	81-91%	89-101%
  EDTA Plasma (n=5)	95-105%	88-98%	80-99%	92-99%
  Heparin Plasma (n=5)	84-98%	95-105%	95-103%	91-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A2 (ANXA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A2 (ANXA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A2 (ANXA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Annexin A2 (ANXA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 125.00-8,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	82-102	95
  EDTA Plasma (n=5)	79-102	95
  Heparin Plasma(n=5)	81-94	85

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A2 (ANXA2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A2 (ANXA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ANXA2 Microplate: 96 Wells (12 x 8 Well strips)
  • ANXA2 Lyophilized Standard: 2
  • 100X Biotinylated ANXA2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 43 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Annexin A2 (ANXA2). No significant cross-reactivity or interference between Annexin A2 (ANXA2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for ANXA2 ELISA kit (Rat) on CiteAb

Target Info

• Gene Symbol: Anxa2
• Gene Full Name: annexin A2
• Alias Symbols: annexin A2;Annexin II;annexin-2;ANX2;calpactin I heavy chain;calpactin-1 heavy chain;chromobindin-8;lipocortin II;p36;PAP-IV;placental anticoagulant protein IV;protein I.
• NCBI Gene Id: 56611
• Protein Name: Annexin A2
• Description of Target: Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9.
• Uniprot ID: Q07936
• Protein Accession #: NP_063970.1
• Nucleotide Accession #: NM_019905.1
• Protein Size (# AA): 339