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Catalog No: OKCD00448 (Formerly GWB-KBBHY8)
Size:96WELLS
Price: $675.00
SKU
OKCD00448
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Datasheets/ManualsPrintable datasheet for ANXA11 ELISA Kit (Human) (OKCD00448)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A11 (ANXA11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A11 (ANXA11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A11 (ANXA11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Annexin A11 (ANXA11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A11 (ANXA11) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A11 (ANXA11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ANXA11 Microplate96 Wells (12 x 8 Well Strips)
ANXA11 Lyophilized Standard2 x 10 ng
100X Biotinylated ANXA11 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Annexin A11 (ANXA11). No significant cross-reactivity or interference between Annexin A11 (ANXA11) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolANXA11
Gene Full Nameannexin A11
Alias Symbols56 kDa autoantigen;ALS23;annexin A11;annexin XI;annexin-11;ANX11;calcyclin-associated annexin 50;CAP50;CAP-50;epididymis secretory sperm binding protein.
NCBI Gene Id311
Protein NameAnnexin A11
Description of TargetBinds specifically to calcyclin in a calcium-dependent manner (By similarity). Required for midbody formation and completion of the terminal phase of cytokinesis.
Uniprot IDP50995
Protein Accession #NP_001148
Nucleotide Accession #NM_001157.2
Protein Size (# AA)505
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