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Catalog No: OKEH03091
Size:96T
Price: $525.00
SKU
OKEH03091
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ANXA1 ELISA Kit (Rat) (OKEH03091) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Anxa1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Anxa1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Anxa1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Component
ComponentAmount
Anti-Anxa1 Microplate96 Wells (12 x 8 Well strips)
Anxa1 Lyophilized Standard2
100X Biotinylated Anxa1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Rat Annexin A1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolAnxa1
Gene Full Nameannexin A1
Alias SymbolsAnnexin 1 (p35) (Lipocortin 1);annexin A1;annexin I;annexin-1;Anx1;calpactin II;calpactin-2;chromobindin-9;lipocortin 1;lipocortin I;p35;phospholipase A2 inhibitory protein.
NCBI Gene Id25380
Protein NameP07150
Description of TargetAnnexin A1
Uniprot IDhttps://www.uniprot.org/uniprot/P07150
Protein Accession #NP_037036.1
Nucleotide Accession #NM_012904.2
Protein Size (# AA)346
Molecular Weight39 kDa
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