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Datasheets/Manuals | Printable datasheet for Anti-Villin 2/Ezrin/p81 Polyclonal Antibody (OAAI00438) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot |
Additional Information | Background: Villin 2, also known as Ezrin, p81, 80K, or cytovillin, is a cytoplasmic peripheral membrane protein which is enriched in microvilli and other cell surface structures. Villin belongs to a member of the gelsolin family of calcium-regulated actin-binding proteins. Unlike the ubiquitously expressed gelsolin, villin expression is restricted to simple epithelia of the gastrointestinal and urogenital tracts. It is localized to the apical cytoplasm and brush borders of these cells. Villin functions in the regulation of actin dynamics in the apical epithelium, capping, nucleating and/or severing actin filaments in a calcium-dependent manner and regulating cell shape in response to external stimuli. Villin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cells. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. |
:: | Positive Control: Hela cells |
:: | Cellular Location: Cytoplasmic/Peripheral membrane |
Reconstitution and Storage | The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles. |
Immunogen | A synthetic peptide corresponding to the C-term portion of human Ezrin protein. This sequence is identical to human, rat, mouse, dog, Monkey, zebra fish, and human origins. |
Purification | Affinity Purified |
Concentration | 100ug/200ul |
Specificity | EZR |
Formulation | This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
Application Info | WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested |
Reference | Krieg,J. and Hunter,T. Identification of the two major epidermal growth factor-induced tyrosine phosphorylation sites in the microvillar core protein Ezrin. J. Biol. Chem. 267 (27), 19258-19265 (1992). |
Gene Symbol | EZR |
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Gene Full Name | ezrin |
Alias Symbols | CVIL;CVL;cytovillin 2;epididymis secretory protein Li 105;ezrin;HEL-S-105;p81;VIL2;villin 2 (ezrin). |
NCBI Gene Id | 7430 |
Description of Target | Villin 2, also known as Ezrin, p81, 80K, or cytovillin, is a cytoplasmic peripheral membrane protein which is enriched in microvilli and other cell surface structures, Villin belongs to a member of the gelsolin family of calcium-regulated actin-binding proteins. Unlike the ubiquitously expressed gelsolin, villin expression is restricted to simple epithelia of the gastrointestinal and urogenital tracts. It is localized to the apical cytoplasm and brush borders of these cells. Villin functions in the regulation of actin dynamics in the apical epithelium, capping, nucleating and/or severing actin filaments in a calcium-dependent manner and regulating cell shape in response to external stimuli. Villin serves as a tyrosine kinase substRate, and is phosphorylated in EGF-stimulated cells. This protein plays a key role in cell surface structure adhesion, migRation and organization, and it has been implicated in various Human cancers. |
Uniprot ID | P15311 |
Molecular Weight | 92.5 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Anti-Villin 2/Ezrin/p81 Polyclonal Antibody (OAAI00438)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "Anti-Villin 2/Ezrin/p81 Polyclonal Antibody (OAAI00438)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Anti-Villin 2/Ezrin/p81 Polyclonal Antibody (OAAI00438)" provided in?
This item is provided in "Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-Villin 2/Ezrin/p81 Polyclonal Antibody (OAAI00438)"?
This target may also be called "CVIL;CVL;cytovillin 2;epididymis secretory protein Li 105;ezrin;HEL-S-105;p81;VIL2;villin 2 (ezrin)." in publications.
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What is the shipping cost for "Anti-Villin 2/Ezrin/p81 Polyclonal Antibody (OAAI00438)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Anti-Villin 2/Ezrin/p81 Polyclonal Antibody (OAAI00438)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Anti-Villin 2/Ezrin/p81 Polyclonal Antibody (OAAI00438)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "92.5".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-Villin 2/Ezrin/p81 Polyclonal Antibody (OAAI00438)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "EZR"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "EZR"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "EZR"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "EZR"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "EZR"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "EZR"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.